Nematodes capture by Arthrobotrys oligospora KTS 1001
– A nematode hungry fungus.
Sandeepa Inamdar Kanitkar and Ravi. U. Kanitkar
Krishi Tantra Sanstha 12, Ganeshwadi Pune 411 004 firstname.lastname@example.org
Arthrobotrys oligospora belongs to a group of nematophagus fungi, which possess specialized structures - in the form of rings, which are extensions of the mycelium to capture, kill and digest nematodes. Arthrobotrys spp
have been reported to be present in many suppressive soils. They are usually found in soils with high organic matter content. They are also commonly found in waste mushroom beds. In India, they can be easily
isolated from soils near cattle-sheds where the excreta of the animals are collected in a common dump before use.
Samples from these sources were collected and plated on Martin’s Rose Bengal agar. Profuse growth of Arthrobotrys was seen in a week in 6 out of 10 samples. After purification, the fungus was
grown water agar with Rose Bengal. Profuse ring formation was observed in a week, after which juveniles of Root Knot nematode were introduced in a plate. Daily microscopic observation of the backside of the
petri-plate was carried out under low power [10x]. Experiment was carried out in triplicates with control plate containing nematodes only. Daily observation was carried out. The number of nematodes captured and
killed was increased from 4th day and seen up to 8th day.. Abundant sporulation was observed from the rings, which had trapped and digested nematodes.