Fluorescence Microscopy for the Observation of Nematophagous Fungi inside Soil, Mycologist Aug.98
Abstract: Fluorochroming of
soil samples makes it possible to study soil fungi in their natural habitat. This is demonstrated with some nematophagous (predacious) fungi.
Nematophagous Fungi - Study by Fluorescence Microscopy and EDX-Technique of the Periodicity of Trap Formation in Soil, Biological Rhythm Research 1997
Abstract: The recently developed method of studying fungi inside soil by fluorescence microscopy was used to examine the periodism of trap formation by predacious fungi.
Therefore, this method was supplemented by a technique, which also permitted serial sections of the soil and the counting of capture organs of Arthrobotrys oliqospora in the soil.
Fluorescence microscopy of fungi in native soil - improvement by additional substances, MICROSCOPY&ANALYSIS 1995
Abstract: The fluorescein - or fluorescein di-acetate respectively - based method
of observation by fluorescence microscopy of fungi inside the soil was improved by application of calcofluor white and rhodamin B. Also the installation of additional filter units to the used Leitz microscope together with special
preparation techniques helped for better exploitation of the method. In addition, the fast photo-fading of the fluorescein could be retarded by citifluor two to three fold.
Differences in the mycelial growth rhythms in a population of Sclerotinia fructigena, in Experientia 39 (1983),
Abstract:
Of 196 strains of Sclerotinia fructigena 181 grew rhythmically in light-dark cycles, while 30 isolates also exhibited rhythms under constant conditions, which indicates an endogenous periodicity. Among the latter 4 strains were
found with well-expressed circadian rhythms. The results are thought to be compatible with an individual genetic creation of rhythms in this population.
The senescence phenomenon in the hyphomycete Monilia fructigena (PERS.) PERS. , (unpublished, 2002)
Summary:
196 strains of Monilia fructigena (also called Sclerotinia fructigena (Pers.) Schröter) isolated for different types of rhythmic behavior also exhibited different ageing. .... These types of senescence are compared to those known from Podospora pauciseta.
Nematophagous Fungi: Guide by Philip Jacobs
So far we know more than 160 fungus species that live on Nematodes partially or entirely
(DOWE 1987). Nematophagous fungi are found in almost every natural soil and aditionally in a number of other substrates. They play an important role as antagonists of nematodes. Therefore there is great interest in research of
possibilities to use them in biological plant protection.
(last changed 30-04-2002)
Nematodes capture by Arthrobotrys oligospora KTS 1001
– A nematode hungry fungus.
Sandeepa Inamdar Kanitkar,
India; committed exclusively for BRIC by the author
Arthrobotrys oligospora belongs to a group of nematophagus fungi ... They are usually found in soils with high organic matter content. In India, they can be easily isolated from soils near cattle-sheds where the excreta of the animals are collected in a common dump before use.
Samples from these sources were collected and plated on Martin’s Rose Bengal agar.
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